Human Anti-HIV-1 gp120 Recombinant Antibody (clone 3bnc117)
CAT#: PABL-163
Recombinant Human Antibody (3bnc117) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Tested Data
Figure 4 (D–F) ELISA assays to determine dual specificity of the 3BNC117/10-1074 biNAb.
(D) Competition ELISA with increasing concentrations of each of the parental bNAbs or a mixture (1:1) of the two bNAbs to determine dual specificity of the biNAb. (E) The 3BNC117/10-1074 biNAb and the 3BNC117 and 10-1074 bNAbs were immobilized to gp140-coated microtiter plates and detected using Fc domain- or light-chain (κ or λ) subclass-specific secondary IgG. The biNAb was detected with both the anti-kappa and anti-lambda secondary antibodies, whereas 3BNC117 and 10-1074 were detected only with anti-kappa or anti-lambda, respectively.
Bournazos, S., Gazumyan, A., Seaman, M. S., Nussenzweig, M. C., & Ravetch, J. V. (2016). Bispecific anti-HIV-1 antibodies with enhanced breadth and potency. Cell, 165(7), 1609-1620.
Figure 5 (G) In vitro neutralization breadth and potency plot of 3BNC117/10-1074 IgG1 biNAb against an extended (120 strain) multiclade virus panel.
Neutralization activity of their respective parental IgG1 bNAbs was included for comparison. The 3BNC117/10-1074 IgG1 biNAb exhibited marked reduction in neutralization potency compared to the activity of a mix of their parental mAbs (determined based on the activity of the most potent parental mAb for a given virus strain)
Bournazos, S., Gazumyan, A., Seaman, M. S., Nussenzweig, M. C., & Ravetch, J. V. (2016). Bispecific anti-HIV-1 antibodies with enhanced breadth and potency. Cell, 165(7), 1609-1620.
Figure 6 In vitro neutralization breadth and potency plots of different anti-Env bNAb expressed as IgG1 or IgG3C- hinge variants. In vitro neutralization was assessed by TZMbl assay against an extended multiclade virus panel.
Bournazos, S., Gazumyan, A., Seaman, M. S., Nussenzweig, M. C., & Ravetch, J. V. (2016). Bispecific anti-HIV-1 antibodies with enhanced breadth and potency. Cell, 165(7), 1609-1620.
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WB
Figure 1 Anti-HIV-1 gp120 Antibody (PABL-163) in WB
Western blot analysis of PABL-163 was performed with HIV-1 gp120 Protein.
ELISA
Figure 2 Anti-HIV-1 gp120 Antibody (PABL-163) in ELISA
ELISA analysis of PABL-163 was performed by coating with HIV-1 gp120 Protein.
DB
Figure 3 Anti-HIV-1 gp120 Antibody (PABL-163) in Dot Blot
Dot Blot analysis of PABL-163 was performed by coating with HIV-1 gp120 Protein.
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Species Reactivity
- HIV-1
- Clone
- 3bnc117
- Applications
- Neut, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA, Neut and TZMbl assay. For details, refer to published data.
Target
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(Creative Biolabs Cat# PABL-163, RRID: AB_3111632)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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